Lysis without lysozym - (Mar/19/2009 )
Dear all
I was told that it should be possible to break up bacterial cells only by freeze/thaw cycles after solving pellet in H2O.
Has anyone tried that ?
pET
-pET-
Don't know about freeze/thaw but I boil the cells in water. That works 
pET on Mar 19 2009, 03:55 PM said:
Dear all
I was told that it should be possible to break up bacterial cells only by freeze/thaw cycles after solving pellet in H2O.
Has anyone tried that ?
pET
I was told that it should be possible to break up bacterial cells only by freeze/thaw cycles after solving pellet in H2O.
Has anyone tried that ?
pET
-T C-