Tissue sonication for RNA extraction - Help :) (Feb/13/2009 )
Help!!!!! I have a question. After using a sonicator in preparation for RNA extraction I achieved results that showed the RNA had been sheared and there was contamination with genomic DNA. Does anyone know why the RNA is damaged yet the DNA is not?????
Thanks
-sazzle-
presence of rnases? maybe not sufficiently inhibited (may explain why sheared rather than gone).
are you talking about shearing of the 28s and 18s rrnas or a smear of mrnas?
a smear of mrnas is normal.
-mdfenko-