MALDI data - (Jan/30/2009 )
Dear all,
I need some help! What software use to analyze MALDI-TOF data?
Thanks in advance
Hey
MALDI-data?
Are you trying to sequence yr protein, if yes then u got to submit it to mascot. We have a GPS explorer software which does it for us. Alternatively take the peak list and submit here:
http://prospector.ucsf.edu/cgi-bin/msform....m=mstagstandard
However, this is just MS based search and u would get better coverage and confidence if you search using both MS and MS/MS data in GPS explorer or protein pilot.
Hope it helps
TC
T C on Jan 31 2009, 05:30 AM said:
MALDI-data?
Are you trying to sequence yr protein, if yes then u got to submit it to mascot. We have a GPS explorer software which does it for us. Alternatively take the peak list and submit here:
http://prospector.ucsf.edu/cgi-bin/msform....m=mstagstandard
However, this is just MS based search and u would get better coverage and confidence if you search using both MS and MS/MS data in GPS explorer or protein pilot.
Hope it helps
TC
Yes only did MALDI-Tof analysis. we want find a phosphorylated residue and decided use MALDI as a first approach... Bad idea?
Thanks for reply
Well
Phosphorylations are tricky....people use dedicated instruments like Qtrap (4000 trap) for analysis of post translational modifications. MALDI can do it, but is difficult. Atleast you would see a difference in masses.
I know people who use MALDI to just see if their in vitro phosphorylation has worked and then use Qtrap. These people are also optimizing conditions. Also, they use IMAC columns to enrinch.
Hope it helps.
TC