Acetone precipitation and SDS removal - (Sep/26/2014 )

Hi to all,

I'd like to perform an acetone precipitation in order to wash high concentrations of SDS (specifically i have a solution of 10% w/v of SDS) from a protein sample. i'm wandering if it is proper to perform the last centrifugation step (after the incubation of the protein sample in acetone @ -20°C) at room temperature in order to reduce the precipitation of SDS with the protein.

Thank you!

(this is a copy of my response at a different forum):

i'm not sure if this is a good method to remove protein from a solution of sds. are you also trying to strip sds from the protein?

acetone precipitation of proteins is much more effective at lower temperatures.

you can remove sds by dialysis (and also strip sds from the protein if you include triton x-100 in the dialysis buffer).

you should also be able to remove protein from sds solution (and from the protein) with methanol.

thank you. what do you mean with remove sds with methanol?

Antonio Sillo Rosato on Fri Sep 26 15:46:37 2014 said:

thank you. what do you mean with remove sds with methanol?

methanol will precipitate protein. it will also keep sds in solution and strip sds from the protein.