Protocol Online logo
Top : New Forum Archives (2009-): : Protein and Proteomics

Heat the sample during nitrogen evaporation of peptides eluted in ethanol/6 N hy - (Apr/03/2014 )

I am doing nitrogen evaporation of a sample of proteins eluted from a sep-pak carteridge in ethanol/6 N hydrochloric acid (1,000:1, v/v). The protein of interest is Oxytocin.

My question is: should I use heat the sample while evaporating? I would think that heating would cause more rapid evaporation, but cold temperature would be safer for protein integrity.. However, will the EtOH/HCL solvent evaporate efficiently without heat??

Any thoughts and comments are greatly appreciated. 

-Nicolai-

oxytocin should be reasonably stable with gentle heating (37C). keep in mind that evaporation will chill the sample so you could also heat to 25C.

-mdfenko-

Hello mdfenko,

 

Thank you for your thoughts on the topic, greatly appreciated!

 

Nicolai

-Nicolai-