Protocol Online logo
Top : New Forum Archives (2009-): : Protein and Proteomics

what kind of acetone should be used for deal with carbon support films - (Dec/18/2013 )

hello everyone,

I want to make protein sample use carbon support films for EM. 

Before using, the films should be dealed with acetone. I do not know which kind of acetone should I use? Something contaminants may be attached onto the film if the acetone grade is not high.

Could you help me? Thank you very much.

 

-Hellen-

when in doubt, use the highest purity grade you can find (hplc or molecular biology grade).

 

first determine if there is a grade specifically recommended for em.

-mdfenko-