Slime formation in DNA extraction - (Mar/13/2013 )
Hallo all,
when I do a DNA extraction I always notice some sort of slime formation at the moment I add 1 buffer and ethanol to the sample.
I see a white precipitate and the slime formation at that point.
I was wondering what this slime is? Is this the DNA?
-lucilius-
Could you be more specific? There are heaps of DNA extraction protocols
-El Crazy Xabi-
El Crazy Xabi on Thu Mar 14 02:38:59 2013 said:
Could you be more specific? There are heaps of DNA extraction protocols
its a qiagen extraction protocol.
You can find the protocol here: http://www.qiagen.com/resources/Download.aspx?id={6B09DFB8-6319-464D-996C-79E8C7045A50}&lang=en&ver=1
Its on page 29, step 3.
Its when you add the ethanol and buffer AL you get the slime.
-lucilius-
It tells you what it is on the top of page 30....
-leelee-
leelee on Thu Mar 14 10:02:20 2013 said:
It tells you what it is on the top of page 30....
No
the white precipitate is something different.
I see that too.
Its not that !
Its a clear (transparant) slimy thing.
-lucilius-
from what organism are the tissues or samples?
-hobglobin-
hobglobin on Thu Mar 14 18:13:58 2013 said:
from what organism are the tissues or samples?
eukaryotic organisms.
unicellular organisms.
-lucilius-
A white precipitate may form on addition of Buffer AL and ethanol. This precipitate
does not interfere with the DNeasy procedure. Some tissue types (e.g., spleen,
lung) may form a gelatinous lysate after addition of Buffer AL and ethanol. In this
case, vigorously shaking or vortexing the preparation is recommended.
I think what you saw is just DNA and is more apparent when DNA concentration is high.
-pcrman-
pcrman on Thu Mar 14 18:21:52 2013 said:
A white precipitate may form on addition of Buffer AL and ethanol. This precipitate
does not interfere with the DNeasy procedure. Some tissue types (e.g., spleen,
lung) may form a gelatinous lysate after addition of Buffer AL and ethanol. In this
case, vigorously shaking or vortexing the preparation is recommended.
I think what you saw is just DNA and is more apparent when DNA concentration is high.
Thats what I think too....
But is there anything known about this? I have seen this before, written down, in for example the DNA extraction of kiwi, but how come DNA forms this white slime?
Altough... the DNA concentration is not that high when I do the extraction.
-lucilius-
You can see DNA even do it in your kitchen http://kitchenpantryscientist.com/?p=2244
-pcrman-