Slime formation in DNA extraction - (Mar/13/2013 )
lucilius on Thu Mar 14 17:16:37 2013 said:
leelee on Thu Mar 14 10:02:20 2013 said:
It tells you what it is on the top of page 30....
No
the white precipitate is something different.
I see that too.
Its not that !
Its a clear (transparant) slimy thing.
They mention both a white precipitate AND a gelatinous lysate....
But anyway, could it be just due to the fact of mixing two different liquids together of different salt conc./densities/etc? For example, when you add an enzyme to a solution, you can see a "slime" (due to the glycerol in the buffer) until you've mixed it properly. It could be something like that?
-leelee-
leelee on Fri Mar 15 01:12:03 2013 said:
lucilius on Thu Mar 14 17:16:37 2013 said:
leelee on Thu Mar 14 10:02:20 2013 said:
It tells you what it is on the top of page 30....
No
the white precipitate is something different.
I see that too.
Its not that !
Its a clear (transparant) slimy thing.
They mention both a white precipitate AND a gelatinous lysate....
But anyway, could it be just due to the fact of mixing two different liquids together of different salt conc./densities/etc? For example, when you add an enzyme to a solution, you can see a "slime" (due to the glycerol in the buffer) until you've mixed it properly. It could be something like that?
It could be yes.
I dont know.
I think its the DNA , but not sure why it forms this slime...
-lucilius-
Lysing your cells sets free its components, membrane macromolecules i.e. glycoproteins which are able to build intermolecular networks causing the formation of gel-like liquid or so-called "slime".
-2xzwei-