DNA polymerase for GC rich template? - (Jan/09/2013 )
In among all the brands of DNA polymerase, which one stand out among all?
I found out Bioline's MyTaq look pretty good when compare to other vendors' DNA polymerase with DNA template of 61%GC.
Anyone uses Bioline's product before?
-badguy-
you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment
-Curtis-
I guess it depends on the target DNA in the end and you have to try out...there is no best one.
-hobglobin-
Curtis on Wed Jan 9 15:27:05 2013 said:
you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment
What Bioline's polymerase you used?
-badguy-
-Curtis-
www.earthox.net
-earthox-