Brief vortexing for reconstitution of recombinant proteins? - (Sep/27/2012 )

Hello,

I have a very simple question.

Generally, vortexing is not recommended while reconsituting lyophilized recombinant proteins.

Can a brief vortexing (e.g. 10 sec. in 1xPBS supplemented with 0.1% BSA) result in a significant loss of the proteins' bioactivity? Did anyone experience problems because of vortexing?

Gunes.


- goals go global -

vortexing can cause denaturation of the protein due to physical stress (and also, possibly, oxidation).

it can be performed if you can cause a swirling effect rather than bumping.