P450 Activity Assay - (Feb/29/2012 )

Hello,


I am working on a bacterial P450 enzyme. I am wondering whether anyone knows of a P450-reductase that can be purchased or the general way to proceed to see activity. I have the enzyme and the substrate but not sure on what type of reductase to use (ferredoxin, cytochrome b?).

here is a p450 reductase (human) sold by sigma.

look at the datasheet under "safety & documentation"