buffer exchange - (Dec/11/2011 )

Hi!

So, I have a purified protein, and I have to exchange the buffer (DTT interfering with downstream binding to the Ni column).

The problem is that I have very small volume (10 uL, 6 uM) of the protein?

Could anyone recommend a type of column to use?
Anyone has experience with low volumes?

Thanks a lot!!

-hannna-

you could use a sephadex g-25 spin column but you will significantly dilute your protein.

you can try drop dialysis.

-mdfenko-

I thought that drop dialysis is used for nucleic acids. At least I use it for that only.

I will try sephadex g 25 column. But I'm still worried about the small volume.. :/

-hannna-

Actually I'm going to order this:
http://www.piercenet.com/browse.cfm?fldID=67687BF8-62AB-4FBD-A404-BEC6E0635687

They say it works with very small volumes.

Does anyone have experaience with it?

-hannna-