reducing agent? - (Aug/05/2011 )

hi

which of the following chemicals is used by you as reducing agent for proteins??

(a) TCEP
( DTT
(c) beta mercaptoethanol

have you found any advantage of one over the other??

They are all used. TCEP supposedly doesn't cause your co-workers to run away (2-ME and DTT smell horribly), however I have not tested it on my precious samples.

Hehe, I use DTT all the time, I really think it smells good, haha.

BioMiha on Fri Aug 5 12:19:27 2011 said:

TCEP always (since i found a really cheap supplier - $300 for 25 gr's compared to ThermoSci's ridiculous pricing)

Main reason is - TCEP is more potent than the others, no odor, stable in air, good with IMAC purification.