brain tissue preparation for immunoblot - (Jun/19/2011 )

how do you dissect brain tissue for immunoblot analyzis, especially if you like to analyze phsophoproteins? I do not mean LASER dissection but with scalpel and binocular... any suggestion is welcome!

I'd freeze it and section with a cryotome then dissect out the bits wanted using a stereomicroscope. Note: I have never done this, but if I had to do it, this is probably how I would go about it.