why phosphorylated protein relative to total protein? - (Mar/28/2011 )

I was wondering, why do i have to calculate the ratio between p-protein relative to total protein? many i have talked to just say: thats how you do wheen you present you data. The reason i ask, wheen i try to get my manuscript published someone in the editorial board points out, i must present my data in this way. But i dont see the advantage to present my data like this. Can you please give me you reflections on this

/ Bettan

bettan on Mon Mar 28 13:54:38 2011 said:

I'm going to assume you are trying to show that a certain Treatment/Condition induces phosphorylation.

The reason to calculate the ratio between p-protein and total protein is to show that your treatment is specifically inducing phosphorylation and that the increase in p-protein is not due to differences in total protein (which could be due to induction of protein expression rather than phosphorylation).

bettan on Mon Mar 28 13:54:38 2011 said:

Hi. Below is a paper describing the different scenarios of phosphorylation status change. Maybe u would find useful:

phosphorylation change-2D gels

Cheers