can i use trypsin to collect cell sample for 2d gel analysis? - (Mar/16/2011 )
can i use trypsin to collect cell line sample from plate for 2d gel proteomic analysis? would that influence much about the result of experiment?
or should i only use a "rubber police"?
thx~
-yimao-
you can use trypsin as long as you wash the cells thoroughly before you lyse them.
-mdfenko-
mdfenko on Wed Mar 16 19:28:38 2011 said:
you can use trypsin as long as you wash the cells thoroughly before you lyse them.
u mean after collect the cell by trypsin, right?
3 time by Wash Buffer good enough?
-yimao-
yimao on Thu Mar 17 03:26:32 2011 said:
u mean after collect the cell by trypsin, right?
yes
3 time by Wash Buffer good enough?
if you drain the pellets well
-mdfenko-