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Problem with dot blot - Denatured sample??? (Jul/25/2010 )

Hello everyone

I would like to ask you if it's necessary a denatured sample to performe dot blot? I have that doubt because many dot blot protocols don't mention it.

I've been doing dot blot to detect SCD-1 from rat liver homogenized without results.

Thanks

-bionike-

bionike on Sun Jul 25 23:22:53 2010 said:


Hello everyone

I would like to ask you if it's necessary a denatured sample to performe dot blot? I have that doubt because many dot blot protocols don't mention it.

I've been doing dot blot to detect SCD-1 from rat liver homogenized without results.

Thanks


what are you dotting (homogenate?) and how do you like to detect SCD-1?

-Inmost sun-