protein absorbance at 230nm??? - (May/06/2010 )
Hi
There are many protocols and methods with 230 nm as the wavelength for measurement of protein. So what contributes to absorbance at 230 nm in protein ???
Do any one has any article justifying the use of 230 nm for protein measurement ???
Thanks a lot
-rick112-
rick112 on May 6 2010, 05:08 PM said:
Hi
There are many protocols and methods with 230 nm as the wavelength for measurement of protein. So what contributes to absorbance at 230 nm in protein ???
Do any one has any article justifying the use of 230 nm for protein measurement ???
Thanks a lot
There are many protocols and methods with 230 nm as the wavelength for measurement of protein. So what contributes to absorbance at 230 nm in protein ???
Do any one has any article justifying the use of 230 nm for protein measurement ???
Thanks a lot
hey rick as far as i know at the wavelength 235 nm protein secondary and tertiary structures are known to absorb light. I have attached a paper too.. hope it helps!!
Good luck!
-Prep!-