Silver stained 2-d gel BLOB!!! - (Apr/22/2010 )
Hi
I was wondering if someone could help me. Everytime I run my protein samples on 2-D gels and silver stain these gels I get this large blob at the base of the gel (see attached picture). I use GE IEF strips and ampholytes and Invitrogen pre-cast gels. I also use Invitrogen LDS sample buffer. I take great care not to touch the gels before staining.
Any help appreciated.
sam
-sam_boooko-
sam_boooko on Apr 22 2010, 11:31 PM said:
Hi
I was wondering if someone could help me. Everytime I run my protein samples on 2-D gels and silver stain these gels I get this large blob at the base of the gel (see attached picture). I use GE IEF strips and ampholytes and Invitrogen pre-cast gels. I also use Invitrogen LDS sample buffer. I take great care not to touch the gels before staining.
Any help appreciated.
sam
I was wondering if someone could help me. Everytime I run my protein samples on 2-D gels and silver stain these gels I get this large blob at the base of the gel (see attached picture). I use GE IEF strips and ampholytes and Invitrogen pre-cast gels. I also use Invitrogen LDS sample buffer. I take great care not to touch the gels before staining.
Any help appreciated.
sam
may be u shud try sum sample clean up kits available in the market!!
-Prep!-