N-Terminal Sequencing - (Mar/24/2010 )

Hi Friends,

I have a doubt regarding the similarity search of the n-terminal sequence. I have done N-terminal sequencing of my protein of my interest and to find out the similar proteins to this I ran BLAST. But the result is very confusing. B'coz, only just 6-7 proteins show identity? will it be sufficient? or is it necessary that the entire stretch have to match? Please help.

Thank you in advance

How many residues of sequence data did you get? What is the quality of the data like?

swanny on Mar 25 2010, 05:47 PM said: