how to extract protein from agarose? - (Dec/30/2009 )
I seeded cells in agarose to let them being mechanical stimulated. Now I want to extract protein from the cells to do iTRAQ analysis to see whether protein expression is changed after mechanical stimulation. Dose anybody know how to extract protein from agarose?
Thank you so much!
-wangjing-
Does anybody know? Help~~~~ Thanks!
-wangjing-