Do you add 10% Glycerin in your IP Lysis Buffer - and do you use 1% NP40 or 1% TritonX-100? (Dec/04/2009 )

It seems that Glycerin can protect the interaction of protein while rotating or something like that by making the solution more sticky??
But will it also reduce the efficiency of antibody-protein interaction as it makes the solution more sticky?
So what is your choice?
By the way,when I say Lysis Buffer,I mean NP-40 Lysis Buffer.
And do you adjust the pH to 7.2 or 7.4 or 8.0?hehe

I haven't used glycerin, I use 0.5% triton X-100 and I always get very good results in IP. by the way I adjust the pH to 7.4

mtrnbh on Dec 7 2009, 01:05 AM said: