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MBD2 and MBD3 ChIP - suitable antibodies needed (Oct/20/2009 )

Hi everyone,

I'm new to this forum and at the moment I'm trying to get ChIP working with antibodies against MBD2 and MBD3 (of the NuRD complex). Has anyone of you tried this and can recommend an antibody? I already searched literature but even with antibodies used in ChIPs I don't get any signal. Is it for example better to leave out the LiCL buffer? Or is there any "trick" regarding this two proteins? :(

Thanks for your help

-SIGNY-

SIGNY on Oct 20 2009, 05:02 AM said:

Hi everyone,

I'm new to this forum and at the moment I'm trying to get ChIP working with antibodies against MBD2 and MBD3 (of the NuRD complex). Has anyone of you tried this and can recommend an antibody? I already searched literature but even with antibodies used in ChIPs I don't get any signal. Is it for example better to leave out the LiCL buffer? Or is there any "trick" regarding this two proteins? ;)

Thanks for your help


Have you gotten ChIP to work with any other antibodies for other proteins, for instance Pol II or histones?

-KPDE-