Why can't genomic DNA be stored at 2-8 for long periods? - (Sep/25/2009 )

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hobglobin on Sep 26 2009, 12:48 PM said:

Bassaml7 on Sep 26 2009, 09:00 PM said:

gleb.kudr on Sep 26 2009, 08:43 AM said:

Bassaml7 on Sep 26 2009, 02:03 AM said:

How about Primers ? Can I also store aliquots of my primers safely at 2-8 at least for a month or so ?

Depend on concentration and primer length. More dilution and less length are both affected primer stability. 10nmol/40 b.p. worked normally after 2 months store at 4C.

Thank you for your reply. My primers are actually about the standard length i.e around 20 bp . I wish I can find a reference Nucleic acids Chemistry from which I can conclude all this kind of information and even understand the detailed mechanisms behind it.

The main risk in storing DNA at 2-8 degrees is that fungi or bacteria can grow if there is any contamination (contaminated solutions or tips,...). Sometimes it happens and you don't know why, so I'd only put aliquots at that temperature and the stock in the freezer.
TE (i.e the EDTA) also helps to inactivate enzymes that cleave DNA.

Thank you very much that's quite helpful. :lol:

-Bassaml7-

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