Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Knockdown Related Discussions
  1. Anyone ever mixed two siRNAs for in vivo knockdown? - (reply: 2)
  2. Protein knockdown method? - (reply: 1)
  3. BLOCK-iT Pol II miR RNAi system: Issues with generating stable knockdown - (reply: 1)
  4. Knockdown with shRNA not stable (Why?) - (reply: 3)
  5. miRNA knockdown in a mouse, tail vein injection (~50% KD) - (reply: 2)
  6. Antibody validation for ChIP - high backgound after knockdown - (reply: 4)
  7. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
  8. Gene knockdown expression levels shRNAmirs - (reply: 2)
  9. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
  10. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
  11. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
  12. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
  13. Little knockdown but big effect on proliferation - (reply: 2)
  14. Knockdown induce apoptosis - (reply: 5)
  15. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
  16. Confirmed gene knockdown not showing on array - (reply: 2)
  17. Phenotypes with two different siRNA but no knockdown? - trouble in showing a knock-down (reply: 2)
  18. miRNA knockdown experiment controls - Very confused (reply: 2)
  19. miRNA knockdown approaches - (reply: 6)
  20. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
  21. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  22. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
  23. Help: protocols for shRNA knockdown effect on Ag-specific Treg differentiation - (reply: 1)
  24. nonreproducible knockdown efficiency with Plemir-micorRNA from Open Biosystem - (reply: 3)
  25. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  26. Importin alpha siRNA - Looking for siRNA for knockdown of all Importin alpha subtypes (reply: 2)
  27. Dicer knockdown - (reply: 6)
  28. How to do stable knock-down by shRNA – advices for beginner - (reply: 1)
  29. Effect of MOI on lentiviral shRNA knockdown - (reply: 1)
  30. Why there is no knockdown? - (reply: 3)
  31. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  32. Lentivial Knockdown of miRNAs - (reply: 8)
  33. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  34. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  35. Problems with gene knockdown - No knockdown with validated shRNAs :( (reply: 6)
  36. Knockdown of AcpP in S. enterica while in macrophages - PubMed link (reply: 1)
  37. stable transfection problems - stable knockdown (reply: 3)
  38. Knockdown of miRNAs - (reply: 8)
  39. RNAi in Zebrafish - vector for zebrafish knock-down (reply: 4)