Which one´s the best TA-Cloning kit?
I´m using the pGEMTeasy kit from Promega but I hope there´s a better one.
Please tell me ...

---

Invitrogen TOPO TA cloning kit always work

---

Agree. I always use TOPO TA cloning kit from IVG.

---

Its true. TOPO TA from Invritrogen always work. Invritrogen has also TA Cloning kit. the main difference is that TOPO TA's ligation is at benchtop for 5-30 min and TA ligation is at 14C overnight. Obviously there are differences in cost, being the TA the most economical. In terms of efficacy, I have heard that TA (overnight ligation) is more efficient. I am an active user of TOPO TA and I can tell you that is VERY GOOD.

Hope this helps

---

I prefer Pstblue 1, Perfectly blunt clonning kits from Novagen. Is quick, easy and high efficiency.
In one day you can do the ligation(20 minutes) reaction and transformation (one hour).

---