Isothermal DNA amplification

Isothermal DNA amplification - a "hot topic" among the local commercial company (Jun/15/2011 )

There are quite a number of Isothermal DNA amplification techniques.
This includes LAMP, HDA, RPA and etc.

Did anyone of you use it here? Do it have any values in your research? Synthetic Biology? Molecular Diagostic?

How do you think?

I had read some publications saying that such technology is better/not good than conventional PCR. But how come people were so phobia about PCR anyway?

-Adrian K-

Adrian K on Wed Jun 15 17:05:06 2011 said:

how come people were so phobia about PCR anyway?

cost of setting up (thermal cycler).

-mdfenko-

here is a bioforum thread were it was a topic already, though not that long....

-hobglobin-

hobglobin on Wed Jun 15 20:33:39 2011 said:

here is a bioforum thread were it was a topic already, though not that long....

Hi Hobglobin, can PM me the paper?

Btw, did anyone had any experience in doing such work before?

-Adrian K-