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Protocols
- OligoAnalyzer (IDT)
Cool tool for oligo analysis. Features include analysis of hairpin, self-dimer, heter-dimer, and other basic parameters.
http://biotools.idtdna.com/analyzer/Applications/O...
Added: Wed Mar 24 2004, Hits: 10307, Reviews: 0 Write review - PCR optimization (Practical Molecular Biology)
The program helps to evaluate quantyty of primers and nucleotides for optimal PCR reaction and the necessary number of cycles.
http://www.molbiol.ru/eng/scripts/01_14.html
Added: Sat Oct 12 2002, Hits: 5036, Reviews: 0 Write review Cached -
Oligo Calculator
(Eugen Buehler, Department of Molecular Genetics and Biochemistry University of Pittsburgh )
Enter an oligo sequence and get various parameters such as Tm, %GC, molecular weight.
http://micro.nwfsc.noaa.gov/protocols/oligoTMcalc....
Added: Sat Jul 20 2002, Hits: 2694, Reviews: 1 Read reviews Write review -
OligoCalc
(Pierre Rodrigues, JustBio)
With this tool you can simply and quickly calculate your primer parameters (Tm, %GC, ...) (Free registration is needed)
http://www.justbio.com/oligocalc/index.php
Added: Sun Aug 18 2002, Hits: 1855, Reviews: 0 Write review Cached -
PCR Box Titration Calculator
(Attotron Biosensor Corporation)
This JavaScript program calculates the amounts of various ingredients needed to do two-dimensional box titrations of various reactants in polmerase chain reactions.
http://www.attotron.com/pub/pcrtitr.htm
Added: Sat Jul 20 2002, Hits: 1513, Reviews: 0 Write review Cached -
Tm Determination
(Breslauer , Virtual Genome Center )
This program caculates primer Tm and assumes that the sequences are not symmetric and contain at least one G or C. The minimum length for the query sequence is 8.
http://alces.med.umn.edu/rawtm.html
Added: Sat Jul 20 2002, Hits: 341, Reviews: 0 Write review Cached