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Top : Molecular Biology : Protein : Western Blotting : Stripping Western Membranes : Stripping Western Membranes
Stripping Western Membranes | |
| Author: Monica Colitti | |
| Source: Contributed by Monica Colitti | |
| Date Added: Tue May 14 2002 | |
| Date Modified: Thu Apr 29 2004 | |
| Abstract: Simple protocol for reusing blot | |
Procedure
- Put the membrane in PBS or TBS with 7ul/ml B-mercaptoetanol and 2%SDS for 30' at RT in agitation
Wash the membrane for 30' in PBS or TBS.
Put the membrane in blocking buffer