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Top : Cell Biology : Cell Culture : General Procedures : Culture Vessels : Recommended working medium, trypsin volume and cell inoculation density
Recommended working medium, trypsin volume and cell inoculation density | |
| Author: Long-Cheng Li | |
| Source: | |
| Date Added: Tue May 14 2002 | |
| Date Modified: Tue Apr 27 2004 | |
| Abstract: Useful data for cell subculture with different culture vessels. | |
Recommended working medium, trypsin volume
and cell inoculation density
(7/9/99)
| Type of Vessels | Culture Area (cm2) | Volume (ml) | Working Medium (ml) | Inoculation Density | Volume of Trypsin (ml) |
| Nunclon Flask | 24 | 50 | 7 | 4.8x105 | 0.5-0.8 |
| Corning Flask | 25 | 70 | 7 | 5x105 | 0.5-0.8 |
| Falcon Flask | 75 | 250 | 25 | 1.5x106 | 1.0 |
| Nunclon Flask | 83 | 260 | 30 | 1.6x106 | 1.2 |
| Nunclon Flask | 175 | 800 | 68 | 3.5x106 | 2.0 |
| Falcon Dish 35x10mm | 9.6 | | 2.5 | 1.6x105 | 0.2-0.3 |
| Falcon Dish 65x15mm | 21 | | 6.0 | 4.2x105 | 0.5-0.6 |
| Falcon Dish 100x20mm | 58 | | 16.0 | 1.1x106 | 1.0 |
| 96 well Plate | 0.32 | | 0.2 | 6x103 | 0.01-0.02 |
| 24 well Plate | 1.88 | | 1.5 | 3.7x104 | 0.08-0.1 |
| 12 well Plate | 3.83 | | 2.0 | 7.6x104 | 0.1-0.2 |
| 6 well Plate | 9.4 | | 3.0 | 1.8x105 | 0.2-0.3 |