Procedure

1. In a single tube, add:
   10 ul chromatin
   85 ul water
   4 ul NaCl (5M)
   8 ul Proteinase K (20 mg/ml)
   4 ul RNase (mg/ml)
   
   Incubate at 65°C for 1 hour.
    
2. Purify with PCR cleanup kit (Qiagen)
3. Spec with Nanodrop
4. Run 200 ng at 90V for 1 hour on a 1% agarose gel with EtBr.

Note

1. In the standard protocols I have found, decrosslinking takes a minimum of 6 hours. For accurate input DNA quantitation, an RNase digest (at least 30 mins) is necessary. Furthermore, a Proteinase K digest is required, adding at least another hour. This adds up to making it unpleasant to do in a single day, so usually it gets done overnight and you lose a day. The above method will let you sonicate, check for size and re-sonicate or IP all in the same day. Run side-by-side, this method yields results nearly identical to the standard protocol in a fraction of the time.
2. It may be important to say we crosslink in 1.8% formaldehyde, aim for 100-200 bp fragments, and in our trials a 10 ul chromatin sample yields 1-2 ug of DNA (We start with primary tissue, so I can't tell you how many cells that corresponds to). Starting with more material may affect the times required for digestion.