Protocols
- How to Make Competent Cells (Sosnick Lab, University of Chicago)
This 6-step protocol describes the CaCl2 method for making competent cells.
http://sosnick.uchicago.edu/competent_cells.html
Added: Tue Aug 13 2002, Hits: 2114, Reviews: 0 Write review Cached - Making Competent Cells For Transformation (Pamela Stanley Lab Wiki, Yale University)
Detailed procedures and recipes for preparing competent cells
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Thu Mar 26 2009, Hits: 21344, Reviews: 0 Write review Cached - Preparation of Electrocompetent Cells and Electroporation of E. coli
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Detailed procedures
http://www.its.caltech.edu/%7Ebjorker/Prep_of_elec...
Added: Wed Aug 14 2002, Hits: 24347, Reviews: 0 Write review -
Cool Growth of Competent E . coli Cells
(Nonet Lab - Washington University School of Medicine)
This protocol is very effective and simple. The fact that the cells are grown at low temperature greatly increase the OD range during which the cells will prep up at high efficiency.
http://thalamus.wustl.edu/nonetlab/ResourcesF/comp...
Added: Sat Nov 01 2003, Hits: 6087, Reviews: 0 Write review Cached -
Electroporation Competent E.Coli Preparation
(Bowtell Lab)
Including procedures for cell preparation and electroporation
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
Added: Tue May 14 2002, Hits: 661, Reviews: 0 Write review Cached -
Making Competent Bacteria For Transformation
(Pamela Stanley Lab Wiki, Yale University)
This protocol yield competent E.coli with a transfromation rate of 1-3 x 10e8 / ug plasmid DNA.
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Thu Mar 26 2009, Hits: 7930, Reviews: 0 Write review Cached -
Preparation of Competent Cells
(BBHelp)
This protocol describes both CaCl2 and DMSO methods for preparing competent cells.
http://web.uct.ac.za/depts/mmi/bbhelp/bac1.html
Added: Tue May 14 2002, Hits: 7483, Reviews: 0 Write review Cached -
Preparation of Competent Cells
(Donis Keller Lab)
Detailed method for competent cell preparation
http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid1...
Added: Tue May 14 2002, Hits: 4779, Reviews: 0 Write review Cached -
Preparation of Competent E. coli Cells
(Waters Lab)
http://www.molbio.princeton.edu/labs/waters/comp_e...
Added: Tue May 14 2002, Hits: 2235, Reviews: 0 Write review Cached -
Preparation of Electrocompetent Bacteria
(Steven Finkbeiner, UCSF)
http://www.gladstone.ucsf.edu/gladstone/files/fink...
Added: Fri Feb 20 2009, Hits: 6036, Reviews: 0 Write review -
Preparing and Using Bacterial Cells for Electroporation
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Detailed protocol for cell preparation and electroporation
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 477, Reviews: 0 Write review Cached