Protocols
-
Five-Minute Transformation
(Chun-Ming Liu, )
Quick E. Coli transformation method. It works!
http://www.plant.dlo.nl/projects/hybtech/Liu/magic...
Added: Tue May 14 2002, Hits: 2926, Reviews: 0 Write review -
Large-scale transformation procedures
(YGAC)
This protocol merges a typical LiAc protocol with the reducing agent component of the One-step method. It gives >103/ ug efficiency for our strain. Recipe is for 800 transformations (90 mls cells).
http://ygac.med.yale.edu/mtn/LS_transf_prot.stm
Added: Tue May 14 2002, Hits: 552, Reviews: 0 Write review Cached -
Standard Transformation Protocol
(Promega)
You can find general and useful protocol for the whole experiment of plasmid cloning from DNA and vector preparation, ligation, transformation, plasimd preparation and transfection...
http://www.promega.com/tbs/tm033/tm033.html#iv0c
Added: Tue May 14 2002, Hits: 4324, Reviews: 0 Write review Cached -
Transformation
(Lazo Lab)
One-step PEG method
http://wheat.pw.usda.gov/~lazo/methods/lazo/oneste...
Added: Tue May 14 2002, Hits: 1095, Reviews: 0 Write review Cached -
Transformation of Competent Cells (RbCl2 Method)
(Goldberg Lab)
Very nice protocol for E. Coli transformation including competent cell preparation and recipes
http://wheat.pw.usda.gov/~lazo/methods/goldberg/co...
Added: Tue May 14 2002, Hits: 1444, Reviews: 0 Write review Cached -
Transformation of E. coli
(Hideaki Shiraishi, Kyoto University)
Method based on H. Inoue, H. Nojima & H. Okayama, Gene 96, 23-28 (1990)
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 3027, Reviews: 0 Write review Cached