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Protocols
  • Agarose-Formaldehyde Electrophoresis (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
    RNA electrophoresis under denaturing conditions in 2.2M formaldehyde is performed according to Maniatis et al., (1982) using the MOPS buffer system. RNA under these conditions is fully denatured and migrates according to the log10 of its molecular weight.
    http://www.nottingham.ac.uk/~mbzspd/methods/Agaros...
    Added: Sat Apr 10 2004, Hits: 727, Reviews: 0  Write review   Cached
  • Denaturing Agarose Gel Electrophoresis of RNA (Ambion)
    The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about RNA yield. A denaturing gel system is suggested because most RNA forms extensive secondary structure via intramolecular base pairing, and this prevents it from migrating strictly according to its size.
    http://www.ambion.com/techlib/append/supp/rna_gel....
    Added: Fri Feb 06 2009, Hits: 17491, Reviews: 0  Write review   Cached
  • RNA analysis on non-denaturing agarose gel electrophoresis (Evrogen)
    Non-denaturing agarose gel electrophoresis is good for checking RNA quality. This protocol is highly recommended for this purpose.
    http://www.evrogen.com/technologies/RNA-electropho...
    Added: Fri Feb 06 2009, Hits: 18146, Reviews: 0  Write review   Cached
  • RNA Gel Electrophoresis (Fero Lab, Fred Hutchinson Cancer Research Center)
    RNA gel electrophoresis for Northern blotting analysis or simple inspection
    http://labs.fhcrc.org/fero/Protocols/RNAgel.html
    Added: Sun Aug 01 2010, Hits: 13320, Reviews: 0  Write review   Cached
  • Denaturing polyacrylamide gel electrophoresis of RNA (Beverly Faulkner-Jones)
    Polyacrylamide gels have enough resolving power to separate fragments differing by only one base pair in size, but their range is ~ 5 to 1000 bp. They are much more difficult to handle than agarose gels.
    http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
    Added: Tue May 14 2002, Hits: 927, Reviews: 0  Write review   Cached
  • Formaldehyde-Agarose Gels (Beverly Faulkner-Jones)
    Agarose gels have a smaller resolving power than polyacrylamide gels but a greater range of separation - from 200 bp to >50 kb using standard gels and electrophoresis equipment. RNAs up to 10 000 kb can be separated in agarose gels using pulsed field gel electrophoresis.
    http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
    Added: Tue May 14 2002, Hits: 938, Reviews: 0  Write review   Cached
  • Gel Electrophoresis of RNA (Beverly Faulkner-Jones)
    great tips on RNA gel electrophoresis.
    http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
    Added: Tue May 14 2002, Hits: 1560, Reviews: 0  Write review   Cached
  • Polyacrylamide Gel Electrophoresis (PAGE) of RNA (Frank Bottone)
    For use withRibonuclease Protection Assay (RPA):
    http://www.unc.edu/~fbottone/protocol/page.html
    Added: Tue May 14 2002, Hits: 5660, Reviews: 0  Write review   Cached
  • Recovery of RNA fragments from polyacrylamide gels (Beverly Faulkner-Jones)
    The best method is the crush and soak method. It can be sued to isolate single-stranded RNA from denaturing gels. The RNA is of high purity but the method is long and inefficient if long transcripts are to be recovered (cf. less than 30% of DNA greater than 3 kb is recovered).
    http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
    Added: Tue May 14 2002, Hits: 328, Reviews: 0  Write review   Cached
  • RNA Electrophoresis (electrophoresis.net)
    Basic things to know about RNA electrophoresis
    http://www.electrophoresis.net/tech_rna.asp
    Added: Sun Jun 06 2004, Hits: 8645, Reviews: 0  Write review  
  • RNA Electrophoresis (Fermentas)
    Protocols for denaturing and non-denaturing gel
    http://www.fermentas.com/techinfo/electrophoresis/...
    Added: Fri Feb 06 2009, Hits: 5595, Reviews: 0  Write review   Cached
  • RNA gel ectrophoresis (eLabProtocols)
    RNA electrophoresis with formaldehyde including recipes of all required buffers
    http://www.elabprotocols.com/viewer/?id=167
    Added: Tue Sep 14 2010, Hits: 3723, Reviews: 0  Write review   Cached