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Protocols
- Protein Expression and Purification Protocol (Sosnick Group, University of Chicago)
From plasmid to protein, the protocol offers detailed guide to this whole procedure.
http://sosnick.uchicago.edu/Protein_Express&Purify...
Added: Sun Mar 28 2004, Hits: 890, Reviews: 0 Write review Cached -
Cleavage of His-Tagged Proteins with Thrombin cleavage sites
(Chazin Lab, Center for Structural Biology, Vanderbilt University)
General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites. Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly.
http://structbio.vanderbilt.edu/chazin/wisdom/labp...
Added: Sat Oct 18 2003, Hits: 1850, Reviews: 0 Write review Cached -
Expression and purification of a P-ATPase in E. coli
(Biological Procedures Online )
Expression of a prokaryotic P-type ATPase in E. coli plasma membranes and purification by Ni2+-affinity chromatography
http://www.biologicalprocedures.com/bpo/arts/1/9/m...
Added: Wed Sep 25 2002, Hits: 1069, Reviews: 0 Write review Cached -
GST Pull-Downs from Drosophila Tissue Culture Cells
(BioProtocol)
Describes preparing, binding to glutathione-agarose beads, pull-down experiment
http://bioprotocol.bio.com/protocolstools/protocol...
Added: Thu Oct 03 2002, Hits: 367, Reviews: 0 Write review Cached -
Isolation of proteins from inclusion bodies
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Describes protein expression and purification
http://www.its.caltech.edu/%7Ebjorker/Isolation%20...
Added: Wed Aug 14 2002, Hits: 2424, Reviews: 0 Write review - Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase (Hikmet Geckil's Lab)
This is a fast, efficient and reproducible recovery procedure for periplasmic L-asparaginase from distinctly related gram-negative bacteria, Escherichia coli, Enterobacter aerogenes and Pseudomonas aeruginosa. As the method uses inexpensive organic solvent hexane and an aqueous salt solution, it is also highly cost-effective in comparison with the currently available techniques used for the release of this enzyme. As hexane is a highly water immiscible organic solvent, it can be removed easily from the top of the aqueous phase by a simple evaporation. Enzyme recoveries up to 3-fold with respect to sonication can be achieved with this system.
Added: Wed Oct 22 2003, Reviews: 0 Write review -
Protein Purification Protocols
(Lamond Lab, University of Dundee)
The following protocols are provided: - Bugbuster to break open bacterial cells
- Purifying His-tagged proteins
- Purifying GST-tagged proteins
http://www.lamondlab.com/LTprotBacteria.htm
Added: Thu May 08 2003, Hits: 749, Reviews: 0 Write review Cached -
Recombinant His-6 RII Purification
(Susan Taylor, University of California at San Diego)
Detailed protocol with typical yield approximately 15 mg per liter of cells
http://pkr.sdsc.edu/html/pk_protocols/h6_rii_proto...
Added: Tue Aug 13 2002, Hits: 269, Reviews: 0 Write review Cached -
Recombinant His-6-Cat Purification
(Susan Taylor, University of California at San Diego)
Use this protocol for most His-6-Proteins including GFP. Typical yield approximately 15 mg per liter of cells
http://pkr.sdsc.edu/html/pk_protocols/h6_cat_proto...
Added: Tue Aug 13 2002, Hits: 214, Reviews: 0 Write review Cached -
Recombinant PKA Catalytic Subunit
(Susan Taylor, University of California at San Diego)
Step by step protocol on recombinant catalytic subunit preparation (cAMP-dependent Protein Kinase A).
http://pkr.sdsc.edu/html/pk_protocols/c_prep_proto...
Added: Tue Aug 13 2002, Hits: 95, Reviews: 0 Write review Cached -
T-Antigen (SV40 T-ag ) Expression and Purification
(Chazin Lab, Center for Structural Biology, Vanderbilt University )
Express and purify T antigen GST-fusion protein. GST-T antigen has a tendency to form inclusion bodies. Several precautions have to be taken, both during expression and in the purification process to avoid aggregation.
http://structbio.vanderbilt.edu/chazin/wisdom/labp...
Added: Fri Oct 17 2003, Hits: 106, Reviews: 0 Write review Cached