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Protocols
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Fluorine labelling of protein
(Chen's Lab)
http://www.biochem.ucl.ac.uk/~chen/protocols/fluoe...
Added: Tue May 14 2002, Hits: 996, Reviews: 0 Write review -
Native Gel Electrophoresis of Proteins
(Proteinchemist.com)
http://www.proteinchemist.com/tutorial/natpage.htm...
Added: Sat Jan 31 2009, Hits: 3221, Reviews: 0 Write review Cached -
Protein (Antibody) Fluorescent Labeling
(Mario Roederer)
Gives detailed protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
http://www.drmr.com/abcon/
Added: Tue May 14 2002, Hits: 1506, Reviews: 0 Write review Cached -
Protein Electrophoresis
(Lamond Lab, University of Dundee)
Two protocols are provided: Stripping blots and silver staining.
http://www.lamondlab.com/LTprotGels.htm
Added: Thu May 08 2003, Hits: 1016, Reviews: 0 Write review Cached -
Protein Electrophoresis
(electrophoresis.net)
The basic theory and principles
http://www.electrophoresis.net/tech_protein.asp
Added: Sun Jun 06 2004, Hits: 3880, Reviews: 0 Write review -
RNase Activity Staining on SDS-PAGE
(Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
A detailed procedure for RNase activity staining on SDS-PAGE including recepes.
http://ag.udel.edu/plsc/faculty/pamgreen/rnase.htm
Added: Sat Oct 25 2003, Hits: 1086, Reviews: 0 Write review Cached -
Semi-Dry Electrophoretic Blotting of Protein
(Hideaki Shiraishi, Kyoto University)
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 1138, Reviews: 0 Write review Cached
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