Summary: Colony PCR is a method used to quickly screen for plasmid inserts directly from E. coli colonies without plasmid preparation. PCR reaction is assembled as standard PCR except the template DNA is bacteria.
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Protocols
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Colony PCR
( Cindy Troxell)
Colony PCR can be used to identify colonies where your favorite gene (yfg) has been replaced with a marker gene by homologous recombination, and to distinguish homologous recombination events from non-homologous. It can also be used to identify colonies from a tetrad that carry a particular gene replacement. This is not a substitute for Southern blotting, but an adjunct.
http://www-rcf.usc.edu/~forsburg/pcr.html
Added: Tue May 14 2002, Hits: 4586, Reviews: 0 Write review Cached -
Colony PCR
(D. Amberg)
It works either for E coli and yeast
http://www.upstate.edu/biochem/amberg/protocols/co...
Added: Tue May 14 2002, Hits: 1774, Reviews: 0 Write review Cached -
Colony PCR Protocol
(Preuss Lab, University of Chicago)
http://preuss.bsd.uchicago.edu/protocols/colony.ht...
Added: Thu Feb 12 2009, Hits: 1440, Reviews: 0 Write review Cached