Top : Molecular Biology : PCR : Multiplex PCR

Summary: Multiplex PCR is a variant of PCR which enabling simultaneous amplification of many targets of interest in one reaction by using more than one pair of primers. Since its first description in 1988 by Chamberlain et al, this method has been applied in many areas of DNA testing, including analyses of deletions, mutations, and polymorphisms, or quantitative assays and reverse transcription PCR. Typically, it is used for genotyping applications where simultaneous analysis of multiple markers is required, detection of pathogens or genetically modified organisms (GMOs), or for microsatellite analyses. Multiplex assays can be tedious and time-consuming to establish, requiring lengthy optimization procedures.

Protocols
  • Multiplex amplification of ancient DNA PDF file (Molecular Ecology Group, Max Planck Institute)
    This method allows amplification of multiple PCR fragments (in our hands at least about 50) in a single PCR, saving DNA extract and work. We found it to work well for both mitochondrial and nuclear DNA. Ideally, a complete mtDNA genome can that way be amplified in just two PCRs.
    http://itd.unair.ac.id/files/ebook2/Multiplex%20am...
    Added: Fri Feb 13 2009, Hits: 10464, Reviews: 0  Write review  
  • Multiplex PCR Protocol PDF file (James O’Donnell)
    http://jimmyodonnell.files.wordpress.com/2012/06/m...
    Added: Sat Feb 16 2013, Hits: 6984, Reviews: 0  Write review  
  • Multiplex PCR: critical parameters and step-by-step protocol. PDF file (Biotechniques)
    A protocol for developing a multiplex PCR assay and suggest ways to overcome commonly encountered problems.
    http://www.biotechniques.com/multimedia/archive/00...
    Added: Sat Feb 16 2013, Hits: 5156, Reviews: 0  Write review  
  • PCR and multiplex PCR guide (Octavian Henegariu, Yale University)
    Detailed guide for PCR optimization, like concentration of various components, cycling conditions, adjuvants and more.
    http://info.med.yale.edu/genetics/ward/tavi/Guide....
    Added: Tue May 14 2002, Hits: 19562, Reviews: 0  Write review   Cached
  • Troubleshooting for PCR and Multiplex PCR (Octavian Henegariu, Yale University)
    http://info.med.yale.edu/genetics/ward/tavi/Trbles...
    Added: Thu Apr 22 2004, Hits: 9075, Reviews: 0  Write review   Cached