Summary: Inverse PCR (IPCR), described by Ochman et al in 1988, is a method for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. Inverse PCR has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements.
Protocols
- Inverse PCR (Gottschling Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/gottschling/General%20Protoc...
Added: Tue Mar 03 2009, Hits: 10222, Reviews: 0 Write review Cached - Inverse PCR for PAC-End Sequencing (WashU-Zebrafish Genome Resources Project)
This protocol is used to generate PCR fragments that contain the ends of PAC inserts that can be sequenced.
http://www.genetics.wustl.edu/fish_lab/frank/cgi-b...
Added: Fri Apr 23 2004, Hits: 2902, Reviews: 0 Write review Cached -
Inverse PCR
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
To Isolate DNA Adjacent To Known Sequence in Genomic DNA
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 800, Reviews: 0 Write review Cached -
Inverse PCR
(Gottschling Lab, Fred Hutchinson Cancer Research Center)
For use with Snyder mTn-lacZ/LEU2 based mutagenesis
http://labs.fhcrc.org/gottschling/General%20Protoc...
Added: Fri Jul 26 2002, Hits: 3843, Reviews: 0 Write review Cached -
Inverse PCR
(Maddock Lab, University of Michigan)
http://www.biology.lsa.umich.edu/research/labs/mad...
Added: Fri Apr 23 2004, Hits: 4516, Reviews: 0 Write review Cached -
Inverse PCR
(Langdale Lab, Department of Plant Sciences, University of Oxford)
Another two methods from the same laboratory are also available at http://dps.plants.ox.ac.uk/langdalelab/protocols/PCR/iPCR_Susie.pdf and http://dps.plants.ox.ac.uk/langdalelab/protocols/PCR/iPCR_Tom.pdf
http://dps.plants.ox.ac.uk/langdalelab/protocols/P...
Added: Wed Feb 11 2009, Hits: 6895, Reviews: 0 Write review -
Inverse PCR and Cycle Sequencing Protocols
(E. Jay Rehm,, Berkeley Drosophila Genome Project)
http://www.fruitfly.org/about/methods/inverse.pcr....
Added: Tue May 14 2002, Hits: 4202, Reviews: 0 Write review Cached