Protocols
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Calculation of Tm for Oligonucleotides
(IDT)
http://www.idtdna.com/program/techbulletins/Calcul...
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Common Conversions of Oligonucleotides
(Fermentas)
http://www.fermentas.com/techinfo/Appendix/appendi...
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Conversion Factors for Metric Prefixes
(Promega)
http://www.promega.com/biomath/default.htm#prefix
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How to Calculate the Melting Temperature of Oligonucleotides
( )
(Genosys)
http://www.genosys.co.uk/oligos/tech_info/tempertu...
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Melting Temperature of Duplex DNA & Oligonucleotides
(Fermentas)
http://www.fermentas.com/techinfo/Appendix/appendi...
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Micrograms of Double-Stranded DNA to Picomoles
(Promega)
http://www.promega.com/biomath/default.htm#ugpmol
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Micrograms of Linear DNA to Picomoles of Ends
(Promega)
http://www.promega.com/biomath/default.htm#pmolend...
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Micrograms per Milliliter of Oligonucleotides (Single-Stranded DNA) to picomoles per microliter
(Promega)
http://www.promega.com/biomath/default.htm#ugmlpmo...
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Molar Ratio of Insert to Vector for Ligations
(Promega)
http://www.promega.com/biomath/default.htm#insert
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Oligonucleotide Calculator
( )
Determine Tm, MW, etc. of your oligonucleotide (requires javascript enabled browser)
http://micro.nwfsc.noaa.gov/protocols/oligoTMcalc....
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Oligonucleotide Melting Temperature Calculations
(Promega)
http://www.promega.com/biomath/default.htm#melt
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Picomoles of Double-Stranded DNA to Micrograms and Nanograms
(Promega)
http://www.promega.com/biomath/default.htm#pmolug
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Picomoles per Microliter of Oligonucleotides (Single-Stranded DNA) to micrograms per milliliter
(Promega)
http://www.promega.com/biomath/default.htm#pmolulu...
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Quantifying Oligonucleotides
(Synthegen)
Due to the very small amounts of oligonucleotide that is often produced through phosphoramidite synthesis, it is not feasible to measure the quantity of an oligonucleotide empirically. Instead, quantitation is performed by measuring the absorbance of light at 260 nm of a solution of oligonucleotide in TE buffer at pH 8.0.
http://www.synthegen.com/technical/protocols/quant...
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Quantitation of Oligonucleotides
(IDT)
Quantitate your oligo by UV Spectrophotometry or polyacrylamide gel
http://www.idtdna.com/program/techbulletins/Quanti...
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Quantitation of Oligonucleotides
(Genosys)
UV Quantitation
http://www.genosys.co.uk/oligos/tech_info/quantita...
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Universal Bases
(IDT)
Universal bases refer to those bases that exhibit the ability to replace any of the four normal bases without significantly destabilizing neighboring base-pair interactions or disrupting the expected functional biochemical utility of the modified oligonucleotide. Oligonucleotides that include a universal base will function as a primer for DNA sequencing or PCR
http://www.idtdna.com/program/techbulletins/Univer...
Added: Tue May 14 2002, Hits: 51, Reviews: 0 Write review Cached