Protocols
- Making T-A cloning Vectors (Chin-Sang Lab, Queen's University, Kingston, ON, Canada)
A method for direct cloning a PCR product, by the T-vector technique. This is cheap and easy way to clone PCR products with A 3’ overhangs.
http://130.15.90.245/t-a_cloning_vectors.htm
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Construction of Homemade 'T-vectors'
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Making T vector from plasmid for TA cloning
http://www.nottingham.ac.uk/~mbzspd/methods/Constr...
Added: Sat Apr 10 2004, Hits: 234, Reviews: 0 Write review Cached -
How to make a T/A vector
(Protocol Online)
http://www.protocol-online.org/biology-forums/post...
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T-Vector Construction
(Delaney Lab at the University of Vermont)
This protocol should work on the basis of the fact that given an equimolar amount of dNTPs in a PCR reaction, Taq DNA polymerase I will add an extra (template-independent) A. If the enzyme is supplied with only dTTP, then an extra T will be added.
http://www.uvm.edu/~tpdelane/lab/protocols/Tvector...
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