Protocols
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cDNA & Genomic DNA Library Screening
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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cDNA Library Screening
(Laurie Lab, University of Virginia Health Science Center)
Conventional protocol for screening phage cDNA library, in great detail.
http://people.virginia.edu/~gwl6s/home.html/Method...
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cDNA Library Screening
(Karl Clark)
http://www.cbs.umn.edu/~kclark/protocols/library.h...
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Colony Hybridization
(Vesicle Trafficking)
http://cmgm.stanford.edu/~vesicle/protocols/hybrid...
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Expression Library Screening
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Procedures include Growing up host bacteria / Expression Library Titration /Plating Phage/ Screening Plaques with AP-Fusion Protein / In vivo Excision
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
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PCR based DNA library screening method
(Methods.info)
This is a very useful method for the one who wants to screen a phage library non-radioactive and do not need too many clones. Up to 5 clones can be obtained usually within one week. However a prerequisite of this method is some knowledge in ?phage handling and a specific sequence you are searching for.
http://www.methods.info/Methods/Library_screening/...
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Screening of cDNA and genomic libraries
(Atle M. Bones)
http://boneslab.bio.ntnu.no/old_root/lambdascreeni...
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Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose
(Donis Keller Lab)
To transfer DNA from bacterial colonies to nylon (or nitrocellulose) membrane so that a library can be screened by hybridization.
http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
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