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Protocols
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Digoxigenin-UTP RNA Labeling
(Frank Bottone Jr.)
http://www.unc.edu/~fbottone/protocol/dig_rna.html
Added: Tue May 14 2002, Hits: 933, Reviews: 0
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E.coli Total RNA Labeling Protocol for Spotted Microarray
(University of Wisconsin E. coli Genome Project)
Describes RNA preparation, labeling and cleaning up.
http://www.genome.wisc.edu/resources/protocols/rna...
Added: Mon Sep 16 2002, Hits: 726, Reviews: 0
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Reverse Transcription and aa-UTP Labeling of RNA
(DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
Protocol for making cDNA with aa-dUTP incorporation and labeling cDNA via chemical coupling of fluorescent dyes.
http://www.microarrays.org/pdfs/aaUTP_RT_Coupling....
Added: Tue May 14 2002, Hits: 807, Reviews: 0
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Reverse Transcription and Labeling of Human RNA
(DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
Protocol for direct incorporation of fluorescent nucleotides into cDNA reverse transcribed from human mRNA.
http://www.microarrays.org/pdfs/HumanRNALabel.pdf
Added: Tue May 14 2002, Hits: 831, Reviews: 0
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