Top : Molecular Biology : Enzymatic Manipulation of Nucleic Acid : Digestion

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• Restriction Enzymes

Protocols

• DNA Sequential Digestion (Practical Approach Online, Oxford University Press)
  Sequential digestion of DNA using two restriction endonucleases
  http://www.oup.co.uk/pdf/pas/9v1-7-2.pdf
  Added: Sat Aug 17 2002, Hits: 2938, Reviews: 0  Write review  
• Restriction Digests of Genomic DNA (CIMMYT Applied Molecular Genetics Laboratory)
  Very nice protocol for large scale digestion experiments, including some calculations, DNA precipitation after digestion.
  http://www.cimmyt.org/english/docs/manual/protocol...
  Added: Fri Aug 02 2002, Hits: 2970, Reviews: 1 Read reviews  Write review  
• Restriction enzyme digestion of DNA (basic method) (Matt Lewis, Department of Pathology, University of Liverpool)
  Covers many aspects of restriction digestion experiments besides a typical protocol, such as how much DNA is needed, double digestion and star activity.
  http://www.methodbook.net/dna/restrdig.html
  Added: Fri Sep 06 2002, Hits: 5392, Reviews: 0  Write review   Cached
• Alphabetic List of Commercial Restriction Endonucleases and Their Isoschizomers (Fermentas)
  http://www.fermentas.com/techinfo/RE/restrcommercr...
  Added: Tue May 14 2002, Hits: 732, Reviews: 0  Write review   Cached
• Cleavage followed by fill-in of 5' overhangs to generate blunt ends. (NEB)
  http://www.neb.com/neb/tech/tech_resource/restrict...
  Added: Tue May 14 2002, Hits: 162, Reviews: 0  Write review   Cached
• Cleavage with two restriction endonucleases that produce blunt ends. (NEB)
  http://www.neb.com/neb/tech/tech_resource/restrict...
  Added: Tue May 14 2002, Hits: 116, Reviews: 0  Write review   Cached
• Cleavage with two restriction endonucleases that produce compatible overhangs. (NEB)
  http://www.neb.com/neb/tech/tech_resource/restrict...
  Added: Tue May 14 2002, Hits: 118, Reviews: 0  Write review   Cached
• Commercial Restriction Endonucleases Sorted by Generated DNA Ends (Fermentas)
  Enzymes Generating 5'-protruding Ends
  Enzymes Generating 3'-protruding Ends
  Enzymes Generating Blunt Ends
  Enzymes Cleaving DNA on Both Sides of Their Recognition Sequence
  http://www.fermentas.com/techinfo/RE/restrcommre_d...
  Added: Tue May 14 2002, Hits: 654, Reviews: 0  Write review   Cached
• DNA Partial Digestion (Practical Approach Online, Oxford University Press)
  Establishment of conditions for restriction endonuclease partial digestion of genomic DNA.
  http://www.oup.co.uk/pdf/pas/9v1-7-4.pdf
  Added: Sat Aug 17 2002, Hits: 1309, Reviews: 0  Write review  
• Isolation of Restriction Fragments from Agarose Gels by Collection onto DEAE Cellulose (Donis Keller Lab)
  Recovery of DNA from agarose gel
  http://hg.wustl.edu/hdk_lab_manual/rdm/rdm10.html
  Added: Tue May 14 2002, Hits: 426, Reviews: 0  Write review   Cached
• Manual Method of Restriction Digestion of Human DNA (Donis Keller Lab)
  For digestion of human genomic DNA
  http://hg.wustl.edu/hdk_lab_manual/rdm/rdm4.html
  Added: Tue May 14 2002, Hits: 610, Reviews: 0  Write review   Cached
• Partial Endonuclease Digestion (Goldberg Lab)
  http://wheat.pw.usda.gov/~lazo/methods/goldberg/pa...
  Added: Tue May 14 2002, Hits: 460, Reviews: 0  Write review   Cached
• Preparation and Restriction Digestion of Human Cells in Agarose Beads (Donis Keller Lab)
  To prepare high molecular weight DNA in a medium with high surface area, to facilitate complete digestion with restriction enzymes.The DNA prepared in agarose beads is also suitable for use in constructing YAC libraries.
  http://hg.wustl.edu/hdk_lab_manual/rdm/rdm7.html
  Added: Tue May 14 2002, Hits: 374, Reviews: 0  Write review   Cached
• Preparation of Bacteriophage Lambda  Concatamers for use as Size Standards in Pulsed-Field Gel (Donis Keller Lab)
  To produce molecular length size standards accurate in the range of 50-1000 kb.
  http://hg.wustl.edu/hdk_lab_manual/rdm/rdm8.html
  Added: Tue May 14 2002, Hits: 334, Reviews: 0  Write review   Cached
• Preparation of High Molecular Weight Human DNA Restriction Fragments in Agarose Plugs (Donis Keller Lab)
  To prepare intact mammalian chromosomal DNA for use in pulsed-field electrophoresis mapping experiments using rare cutting restriction enzymes.
  http://hg.wustl.edu/hdk_lab_manual/rdm/rdm6.html
  Added: Tue May 14 2002, Hits: 293, Reviews: 0  Write review   Cached
• Procedure for Altering Cleavage Specificities using Methylases (NEB)
  http://www.neb.com/neb/tech/tech_resource/modifyin...
  Added: Tue May 14 2002, Hits: 42, Reviews: 0  Write review   Cached
• Restriction digestion (Roe, OU)
  http://www.genome.ou.edu/protocol_book/protocol_pa...
  Added: Tue May 14 2002, Hits: 1197, Reviews: 0  Write review   Cached
• Restriction Digestion of DNA (Lazo Lab)
  tips on restriction digestion
  http://wheat.pw.usda.gov/~lazo/methods/lazo/rdiges...
  Added: Tue May 14 2002, Hits: 919, Reviews: 0  Write review   Cached
• Restriction Digestion of Plasmid, Cosmid, and Phage DNAs (Donis Keller Lab)
  This is a generalized example of a restriction digest. Estimate the amount of DNA needed in your digest and scale up accordingly. To visualize a digest on an ethidium bromide-stained agarose gel, you will need to take the size of the fragments and the total size of the clone DNA into account (e. g. 10-50 ng of intact lambda-sized genomes (~50 kb) are easily seen on gels but if cut into small (~1kb fragments), the relative proportion of the clone DNA in each fragment is ~ 1/50 and more DNA (500-1000 ng) should be loaded in order to see them).
  http://hg.wustl.edu/hdk_lab_manual/rdm/rdm3.html
  Added: Tue May 14 2002, Hits: 661, Reviews: 0  Write review   Cached
• Restriction Disgestion: Basic Procedures (LaboratoryExperiments.com)
  Basic procedures for high school students, But very useful if you are new to molecular biology.
  http://www.laboratoryexperiments.com/LAB3.htm
  Added: Tue May 14 2002, Hits: 1033, Reviews: 0  Write review   Cached
• Restriction Enzyme Buffer Recipes (Lazo Lab)
  Prepare your customized buffer for a wide variety of restriction enzymes
  http://wheat.pw.usda.gov/~lazo/methods/lazo/restri...
  Added: Tue May 14 2002, Hits: 348, Reviews: 0  Write review   Cached
• Restriction Enzyme Buffers (Donis Keller Lab)
  Buffer constitution for various enzymes
  http://hg.wustl.edu/hdk_lab_manual/rdm/rdm2.html
  Added: Tue May 14 2002, Hits: 508, Reviews: 0  Write review   Cached
• Restriction enzyme digestion of DNA (Julie B. Wolf, University of Maryland, Baltimore County)
  http://www.research.umbc.edu/~jwolf/m3.htm#restric...
  Added: Tue May 14 2002, Hits: 1484, Reviews: 0  Write review   Cached
• Restriction Enzyme Digests (Donis Keller Lab)
  Tips for digestion
  http://hg.wustl.edu/hdk_lab_manual/rdm/rdm1.html
  Added: Tue May 14 2002, Hits: 960, Reviews: 0  Write review   Cached