Protocols
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Methylene Blue DNA staining
(Gerard Lazo)
This method primarily eliminates the damage of DNA by uv irradiation. DNA isolated from methylaene blue stained gels should transform frozen competent E. coli cells on the order of 20-50 fold more efficiently than ethdium bromide isolated DNA.
http://wheat.pw.usda.gov/~lazo/methods/lazo/met1.h...
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