Protocols
- Ancient DNA (aDNA) Extraction from Bones for PCR (Nucleic Acids Research)
A simple and effective modified ethanol precipitation-based protocol is described for the preparation of DNA from ancient human bones. This method is fast and requires neither hazardous chemicals nor special devices. After the powdering and incubating of the bone samples Dextran Blue was added as a carrier for removing the PCR inhibitors with selective ethanol precipitation. This method could eliminate the time-consuming separate decalcification step, dialysis, application of centrifugation-driven microconcentrators and the second consecutive PCR amplification.
http://nar.oxfordjournals.org/cgi/content/full/28/...
Added: Fri Feb 13 2009, Hits: 4785, Reviews: 0 Write review Cached -
Ancient DNA extraction from bones and teeth
(Molecular Ecology Group, Max Planck Institute)
This method is designed to maximize recovery of PCR-amplifiable DNA from ancient bone and teeth specimens and at the same time to minimize co-extraction of substances that inhibit PCR. This is achieved by a combination of DNA extraction from bone powder using a buffer consisting solely of EDTA and proteinase K, and purification of the DNA by binding to silica in the presence of high concentrations of guanidinium thiocyanate.
http://www.eva.mpg.de/molecular-ecology/pdf/aDNA_e...
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Multiplex amplification of ancient DNA
(Molecular Ecology Group, Max Planck Institute)
This method allows amplification of multiple PCR fragments (in our hands at least about 50) in a single PCR, saving DNA extract and work. We found it to work well for both mitochondrial and nuclear DNA. Ideally, a complete mtDNA genome can that way be amplified in just two PCRs.
http://itd.unair.ac.id/files/ebook2/Multiplex%20am...
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Nondestructive DNA extraction method for mitochondrial DNA analyses of museum specimens
(Molecular Ecology Group, Max Planck Institute)
A method that allows extracting DNA from specimens at least 160 years old. It works for mitochondrial DNA and nuclear DNA.
http://www.eva.mpg.de/molecular-ecology/pdf/Non_de...
Added: Fri Feb 13 2009, Hits: 2483, Reviews: 0 Write review