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Protocols
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GST Fusion Protein Preparation
(Vesicle Trafficking)
Detailed protocol for GST protein purification.
http://cmgm.stanford.edu/~vesicle/protocols/gst_pr...
Added: Tue May 14 2002, Hits: 4503, Reviews: 0 Write review Cached -
GST Fusion Protein Purification from Yeast
(Dohlman Lab)
http://www.med.unc.edu/~hdohlman/gst.html
Added: Tue May 14 2002, Hits: 893, Reviews: 0 Write review Cached -
Performing a Hunt by Interaction Mating
(Finley Lab, Wayne State University, School of Medicine)
In this protocol one strain is transformed with library DNA and the transformants are collected and frozen in aliquots. For each interactor hunt, an aliquot of this frozen "pretransformed library strain" is thawed and mixed with an aliquot of a bait strain transformed with the bait expression plasmid. Overnight incubation of the mixture on a YPD plate results in mating - i.e., cells of one strain fuse with cells of the other strain to form diploids. The diploids are then exposed to galactose to induce expression of the library encoded proteins, and interactors are selected in the same manner as in the standard hunt protocols.
http://proteome.wayne.edu/Matinghunt.htm
Added: Tue May 14 2002, Hits: 189, Reviews: 0 Write review Cached -
Purification of GST Fused Proteins
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 201, Reviews: 0 Write review Cached -
Purification of MBP (maLTose-Binding Proteins) Fused Proteins
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 73, Reviews: 0 Write review Cached -
Two Hybrid System Transformation
(Gietz Lab)
This Protocol is for 2 Hybrid system screens
http://www.umanitoba.ca/faculties/medicine/biochem...
Added: Tue May 14 2002, Hits: 1867, Reviews: 0 Write review Cached -
Yeast Two-Hybrid Screen with Library and Bait
(Mirmira Laboratory at the University of Virginia)
http://faculty.virginia.edu/mirmira/resources_file...
Added: Sat Jun 05 2004, Hits: 3726, Reviews: 0 Write review Cached -
Yeast Two-Hybrid System
(Finley Lab, Wayne State University, School of Medicine)
This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with introduction to its background. The following procedures are described: mating assay - small scale for tens of different bait or prey proteins, collecting bait (and prey) strains, large scale for hundreds of different bait or prey strains, interaction mating assay with other yeast two-hybrid systems, recording results, interpreting interaction data, qualitative interpretation and more...
http://proteome.wayne.edu/YTHnetworks.html
Added: Tue May 14 2002, Hits: 8565, Reviews: 0 Write review Cached -
Yeast Two-Hybrid System
(Finley Lab, Wayne State University, School of Medicine)
The updated version for scaling up the these techniques to facilitate characterization of large networks of interacting proteins. It is also useful for individual hunts.
http://proteome.wayne.edu/Update.html
Added: Tue May 14 2002, Hits: 4002, Reviews: 0 Write review Cached