Protocols
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Filter Assay for the Detection of Yeast Colonies Producing §-Galactosidase
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 115, Reviews: 0 Write review Cached -
Isolation of Shuttle Vectors from Yeast and Transformation into E. coli
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Liquid Sporulation
(Herskowitz Lab)
http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
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Long-Term Storage of Yeast Stocks
(Donis Keller Lab)
Yeast storage and revival, two methods are given
http://hg.wustl.edu/hdk_lab_manual/yeast/yeast2.ht...
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Sporulation
(Gottschling Lab, Fred Hutchinson Cancer Research Center )
Sporulation and vivisection
http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
Added: Fri Jul 26 2002, Hits: 1411, Reviews: 0 Write review Cached -
Sporulation Protocol
(Saccharomyces Genome Deletion Project, Stanford)
Efficient sporulation of BY4743 requires growth as a patch on freshly prepared GNA presporulation plates at 30°C overnight prior to transfer to liquid sporulation medium.
http://www-sequence.stanford.edu/group/yeast_delet...
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Streaking Yeast Stocks
(Donis Keller Lab)
Very nice protoocol for yeast work
http://hg.wustl.edu/hdk_lab_manual/yeast/yeast1.ht...
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Yeast Freezedown Guidelines
(Saccharomyces Genome Deletion Project, Stanford)
http://sequence-www.stanford.edu/group/yeast_delet...
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Yeast Sporulation
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
http://labs.fhcrc.org/hahn/Methods/genetic_meth/ye...
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Yeast Sporulation in Liquid Media
(Corbett Lab)
http://www.biochem.emory.edu/labs/acorbe2/yeastspo...
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YPD-filled microtiter plates
(Linda Riles, (Saccharomyces Genome Deletion Project, Stanford)
For growing individual strains to use for PCR
and shipping strains
http://sequence-www.stanford.edu/group/yeast_delet...
Added: Tue May 14 2002, Hits: 1026, Reviews: 0 Write review Cached