Top : Model Organisms : Yeast : Techniques of Yeast Genetics

Protocols

• Beta GAL Filter Assay (Breeden Lab)
  
  http://www.fhcrc.org/labs/breeden/Methods/b-GAL_fi...
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• Beta-gal Assay (Gottschling Lab, Fred Hutchinson Cancer Research Center)
  http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
  Added: Fri Jul 26 2002, Hits: 699, Reviews: 0  Write review   Cached
• Beta-gal Lift Protocol (Dohlman Lab)
  This method is used to screen for and examine mutants with altered pheromone responses.
  http://www.fhcrc.org/labs/breeden/Methods/b-GAL_fi...
  Added: Tue May 14 2002, Hits: 533, Reviews: 0  Write review   Cached
• Bgal filter assay (Herskowitz Lab)
  http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
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• Bgal liquid assay (Herskowitz Lab)
  http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
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• Bgal plate overlay assay (Herskowitz Lab)
  This assay is semiquantitative. Cells can even be recovered from underneath the overlaid agar. The method could be used for any enzyme with a colorimetric assay.
  http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
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• Curing strains of endogenous 2-micron ( )
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 172, Reviews: 0  Write review   Cached
• EMS Mutagenesis (Herskowitz Lab)
  
  http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
  Added: Tue May 14 2002, Hits: 885, Reviews: 0  Write review  
• EMS mutagenesis of yeast (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
  
  http://labs.fhcrc.org/hahn/Methods/genetic_meth/em...
  Added: Tue May 14 2002, Hits: 780, Reviews: 0  Write review   Cached
• Generating a Temperature Sensitive Allele (Herskowitz Lab)
  
  http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
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• Genome-wide Gene Expression Analysis (Richard Young Research Group,Whitehead Institute for Biomedical Research)
  A genoe-wide gene expression analysis using high-density oligonucleotide arrays
  http://web.wi.mit.edu/young/expression/tech.html
  Added: Tue May 14 2002, Hits: 685, Reviews: 0  Write review  
• Handling and using insertion libraries (YGAC)
  http://ygac.med.yale.edu/mtn/protocol.stm
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• Liquid Beta-gal Assays  (Dohlman Lab)
  This protocol is used to quantitatively measure the pheromone response over a range of pheromone concentrations.
  http://www.med.unc.edu/~hdohlman/bgal.html
  Added: Tue May 14 2002, Hits: 548, Reviews: 0  Write review   Cached
• mTn-3xHA/GFP ( )
  insertion library
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 190, Reviews: 0  Write review   Cached
• mTn-3xHA/GFP ( )
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 187, Reviews: 0  Write review   Cached
• mTn-3xHA/lacZ ( )
  insertion library
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 164, Reviews: 0  Write review   Cached
• mTn-3xHA/lacZ ( )
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 148, Reviews: 0  Write review   Cached
• mTn-4xHA/lacZ ( )
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 146, Reviews: 0  Write review   Cached
• mTn-lacZ/LEU2 ( )
  insertion library
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 158, Reviews: 0  Write review   Cached
• mTn-lacZ/LEU2 ( )
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 164, Reviews: 0  Write review   Cached
• Mutagenizing a yeast gene with an mTn (YGAC)
  http://ygac.med.yale.edu/mtn/protocol.stm
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• Non-technical overview ( )
  http://ygac.med.yale.edu/mtn/non_technical_overvie...
  Added: Tue May 14 2002, Hits: 197, Reviews: 0  Write review   Cached
• Preparation of Yease Cell for ImmunoEletron Microscopy (Jon Mulholland, Botstein Lab)
  http://genome-www.stanford.edu/group/botlab/protoc...
  Added: Tue May 14 2002, Hits: 509, Reviews: 0  Write review  
• Protocols for shuttle mutagenesis/epitope-tagging of a yeast gene with mTn-3xHA/lacZ (Yale Yeast Analysis Center)
  Same protocol as above
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 188, Reviews: 0  Write review   Cached
• Protocols for shuttle mutagenesis/epitope-tagging of a yeast gene with mTn-lacZ/LEU2 (Yale Yeast Analysis Center)
  mTn-lacZ/leu2 can easily be inserted at mutiple sites in a given gene. The mutagenized DNA is then transformed into yeast, where it replaces the chromosomal locus by homologous recombination. The transposon insertions create a pool of insertion/disruption alleles. Insertions that generate in-frame fusion of the coding region to lacZ can be used to monitor and quantify gene expression, via assays for beta-gal activity. The fusion protein can also be immunodetected using antibodies directed against beta-gal.
  http://ygac.med.yale.edu/mtn/reagent/avail_reagent...
  Added: Tue May 14 2002, Hits: 334, Reviews: 0  Write review   Cached

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