Top : Model Organisms : Yeast : Protein

Protocols

• Extracts for Yeast Westerns (Herskowitz Lab)
  http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
  Added: Tue May 14 2002, Hits: 6387, Reviews: 0  Write review  
• Preparation of Yeast Protein Extracts (Stephens Lab, University of Bristol)
  http://www.bch.bris.ac.uk/staff/stephens/yeastprot...
  Added: Thu May 27 2004, Hits: 251, Reviews: 0  Write review   Cached
• Quick Total Yeast Protein Isolation for SDS-PAGE Gels (Dohlman Lab)
  A quick method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting (same protocol as above)
  http://www.med.unc.edu/~hdohlman/proteinprep.html
  Added: Tue May 14 2002, Hits: 5565, Reviews: 0  Write review   Cached
• Rapid Protein Preparation (Gottschling Lab, Fred Hutchinson Cancer Research Center)
  Glass bead method
  http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
  Added: Fri Jul 26 2002, Hits: 4010, Reviews: 0  Write review   Cached
• Small Scale Yeast Whole Cell Extract for IP (Hahn Lab, Fred Hutchinson Cancer Research Center)
  http://labs.fhcrc.org/hahn/Methods/biochem_meth/ye...
  Added: Thu Mar 26 2009, Hits: 2585, Reviews: 0  Write review   Cached
• Sucrose Density Gradient Fractionation of Yeast Membranes (Dohlman Lab)
  This is an excellent protocol for localization of membrane associated proteins in yeast.
  http://www.med.unc.edu/~hdohlman/sucrose.html
  Added: Tue May 14 2002, Hits: 1641, Reviews: 0  Write review   Cached
• Whole Cell Extracts (Henrik G. Dohlman, The University of North Carolina Chapel Hill)
  This is a quick method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting.
  http://www.med.unc.edu/%7Ehdohlman/proteinprep.htm...
  Added: Tue May 14 2002, Hits: 3641, Reviews: 0  Write review   Cached
• Yeast Ethanol Lysates for SDS-PAGE and Western Blotting (Markus Ralser)
  This protocol describes a quick method for preparing yeast ethanol lysates for SDS page and western blotting
  Added: Mon Feb 02 2009, Reviews: 0  Write review  
• Yeast Histone Prep (Gottschling Lab, Fred Hutchinson Cancer Research Center)
  http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
  Added: Fri Jul 26 2002, Hits: 1204, Reviews: 0  Write review  
• Yeast Lysates for Western (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 316, Reviews: 0  Write review   Cached
• Yeast Nuclear Extract Prep (small scale) (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
  http://labs.fhcrc.org/hahn/Methods/biochem_meth/po...
  Added: Tue May 14 2002, Hits: 1277, Reviews: 0  Write review   Cached
• Yeast Nuclei Isolation (Hahn Lab, Fred Hutchinson Cancer Research Center)
  This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when the nuclei are salt extracted and the resulting proteins AMSO4 precipitated. The high spermidine and detergent in nuclei isolation buffer are important for isolation of intact nuclei free of cellular debris.
  http://labs.fhcrc.org/hahn/Methods/biochem_meth/ye...
  Added: Thu Mar 26 2009, Hits: 2281, Reviews: 0  Write review   Cached
• Yeast Protein Isolation (Amberg Lab)
  Yaffe-Schatz method
  http://www.upstate.edu/biochem/amberg/protocols/ye...
  Added: Tue May 14 2002, Hits: 1480, Reviews: 0  Write review   Cached
• Yeast Protein Preparation for SDS PAGE and Western (Haha Lab)
  Yeast whole cell protein extraction for western blot using glass bead method
  http://labs.fhcrc.org/hahn/Methods/biochem_meth/ye...
  Added: Tue May 14 2002, Hits: 2812, Reviews: 0  Write review   Cached
• Yeast Protein Preparation for SDS PAGE and Western (rapid) (Haha Lab)
  
  http://labs.fhcrc.org/hahn/Methods/biochem_meth/ye...
  Added: Tue May 14 2002, Hits: 3291, Reviews: 0  Write review   Cached
• Yeast Whole Cell Extract (small scale) (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
  http://labs.fhcrc.org/hahn/Methods/biochem_meth/po...
  Added: Tue May 14 2002, Hits: 2597, Reviews: 0  Write review   Cached